Genome?wide occupancy of <i>Arabidopsis</i> SWI/SNF chromatin remodeler SPLAYED provides insights into its interplay with its close homolog BRAHMA and Polycomb proteins

SPLAYED (SYD) is a SWItch/Sucrose Non-Fermentable (SWI/SNF)-type chromatin remodeler identified in Arabidopsis thaliana (Arabidopsis). It believed to play both redundant and differential roles with its closest homolog BRAHMA (BRM) diverse plant growth development processes. To better understand how SYD functions, we profiled the genome-wide occupancy of impact on global transcriptome trimethylation histone H3 lysine 27 (H3K27me3). map SYD, generated GFP-tagged transgenic line used it for immunoprecipitation experiments followed by next-generation sequencing, which more than 6000 target genes were identified. Through integrating profiles, found that preferentially targets nucleosome-free regions expressed genes. Further analysis revealed peaks exhibit five distinct patterns, also shared BRM BAF60, conserved SWI/SNF complex component, indicating common sites these remodelers functional relevance such patterns. investigate interplay between Polycomb-group (PcG) proteins, performed H3K27me3 syd-5. We observed increases decreases levels at few hundred syd-5 compared wild type. Our results imply can act antagonistically or synergistically PcG specific Together, our data profiles provide much-needed resource dissecting SYD’s crucial regulation development.